Efficient Expression of the Mosquito Larvicidal Binary Toxin Gene from Bacillus sphaericus in Escherichia coli

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Harry Towbin, T. Staehelin, J. Gordon · Proceedings of the National Academy of Sciences · 1979 · 53.8K citations · Full text

Life Sciences, Biochemistry, Molecular Biology +13

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

High efficiency transformation of E.coli by high voltage electroporation

William J. Dower, Jeff F. Miller, Clifton W. Ragsdale · Nucleic Acids Research · 1988 · 2.8K citations · Full text

Life Sciences, Biotechnology, Microbiology +13

Probit Analysis (3rd ed).

J. A. Lewis, D Finney · Journal of the Royal Statistical Society Series C (Applied Statistics) · 1972 · 2.7K citations

Social Sciences, Economics, Financial Economics +4

Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system

F. William Studier · Journal of Molecular Biology · 1991 · 806 citations

Life Sciences, Microbiology, Molecular Biology +13