Decrease in cerebral blood flow in rats after experimental subarachnoid hemorrhage: a new animal model.

Abstract

There continues to be a need for good animal models of experimental subarachnoid hemorrhage (SAH). The rat would be an ideal subject in which to study SAH since it is inexpensive and easier to use than the larger laboratory animals. The present study was undertaken to determine if alterations of cerebral blood flow could be produced in the rat after experimental SAH, and thereby justify using the rat as a model for further study of SAH. Rats weighing between 450 and 500 grams underwent insertion of a cannula into the cisterna magna at least 5 days prior to physiological testing. One group of rats then received a 0.3 cc injection of fresh autologous arterial blood into the cisterna magna to simulate a SAH. Another group of rats received injection of an equal volume of mock CSF (buffered saline) into the cisterna magna. A third group of rats had no subarachnoid injections. In all three groups, blood flow to the cerebral hemispheres was measured with the labeled microsphere technique. Rats with experimental SAH showed a 40% decrease of cerebral blood flow, whereas rats with saline injections showed only a 15% decrease. Control rats had no changes of cerebral blood flow. These studies demonstrate that the rat is a potential experimental model for investigations into SAH.