Proceedings of the National Academy of Sciences · 1976 · 511 citations · 13 references
Life SciencesCell BiologyMolecular BiologyCell AdhesionCell DifferentiationMorphogenesisAnimal Cell CultureCell MigrationCell Shape ChangesCell Shape RegulationContact InhibitionMicroscopic Cell MorphologyAdhesion-dependent Cell MigrationAdhesion-mediated Shape ControlAdhesion-motility CouplingCell Migration MechanismsContact Inhibition Of MotilityContact-dependent InhibitionImpaired Cell AdhesionMorphological Changes During Growth
The isolated chick‑embryo fibroblast glycoprotein CSP was added to various transformed cell lines in vitro. CSP treatment caused transformed cells to elongate, flatten, adhere more strongly, align in parallel arrays, and reduce nuclear overlap—signs of restored contact inhibition—effects that were blocked by anti‑CSP antibody, did not change cAMP levels or growth rates, and thus suggest CSP regulates adhesion and morphology rather than proliferation.
We have isolated the major cell surface glycoprotein of chick embryo fibroblasts, CSP, and added it to a variety of transformed cells in vitro. The transformed cells become more elongated, often more flattened, and show increased adhesion to the substratum. Several transformed cell lines also align in striking parallel arrays. This alignment is characterized by a decrease in the amount of nuclear overlapping, probably indicating restoration of contact inhibition of movement. The morphological changes are antagonized by antibody to CSP. These effects of CSP are not associated with an elevation of cellular 3':5'-cyclic AMP. Moreover, the morphological reversion is not accompanied by an alteration in growth properties. Our results are consistent with a role for CSP in cell adhesion and morphology but not in growth control.
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