Transforming growth factor β expression in human marrow stromal cells

Abstract

The effects of hematopoietic cytokines on the expression of transforming growth factors (TGF beta) mRNA and the effect of TGF beta on cytokine and on a major extracellular matrix protein, collagen I, mRNA expression was studied in human marrow stromal cells. As with other cultured mesenchymal cells, stromal cells constitutively express TGF beta 1 but not TGF alpha mRNA. In simian virus 40 (SV40)-transformed stromal cells downregulation of TGF beta 1 expression was observed 2 hours after incubation with recombinant human (rh) tumor-necrosis factor alpha (TNF alpha) and 144 h after addition of rh granulocyte macrophage colony-stimulating factor (GM-CSF). Neither interleukin-1 (IL-1)beta nor IL-6 had an observable effect on TGF beta 1 mRNA expression. TGF beta upregulated collagen I mRNA expression. These data suggest that cytokines may influence TGF beta mRNA expression.