Preparation and properties of liposome-associated gentamicin

Abstract

Gentamicin was successfully incorporated into neutral, anionic, and cationic liposomes, and the percentage of gentamicin incorporated was found to be a function of lipid concentration. Gentamicin did not leak out of the liposomes over a 3-week period at 4 degrees C. When liposome-associated gentamicin was administered intravenously to rabbits, its serum half-life was greatly prolonged. Intragastric administration of dipalmitoylphosphatidylcholine liposomes containing gentamicin resulted in the appearance of gentamicin in serum. Liposome-associated gentamicin, when administered intravenously, led to the appearance of gentamicin in the liver and spleen, which was not observed when rabbits were injected with free gentamicin.