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PURIFICATION OF GONADOTROPHIN FROM HUMAN MENOPAUSAL URINE
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1964
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SpermatogenesisFertilityGlycobiologyGynecologyFemale Reproductive FunctionReproductive BiologyReproductive EndocrinologyReproductive PhysiologyFemale InfertilityBioanalysisAbstract GonadotrophinsPublic HealthChromatographyInfertilityAndrologyBiochemistryKaolin-acetone MethodEndocrinologyOvarian HormoneUrologyMenopauseMedicineEndocrine ResearchPurified HmgReproductive Hormone
ABSTRACT Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C). The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity. The immunoelectrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region α 1 , α 2 and β globulins.