European Journal of Endocrinology · 1964 · 37 citations · 0 references
Life SciencesBiochemistryEndocrinologyHormone BiochemistryReproductive EndocrinologySteroid BiochemistryGonadal HormonesProtein PurificationSpecific Activity Determination
ABSTRACT Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C). The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity. The immunoelectrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region α 1 , α 2 and β globulins.