Marine Ecology Progress Series · 1985 · 44 citations · 7 references
Life SciencesMarine SciencesMicrobiologyMarine MicrobiologyMicrobial MetabolismMicrobial PhysiologyAquatic Microbial EcologyCell Growth KineticsMarine Microbial MetabolismMarine Microbial PhysiologyMicrobial BiogeochemistryMicrobial GrowthChemostat CultureLow-temperature GrowthMicrobial Growth KineticsMicrobial KineticsOptimum TemperatureTemperature Effects On Cell GrowthTemperature Effects On GrowthTemperature Optimum
An ammonium oxidizing bacterium has been isolated from Alaskan waters that is capable of growth at -5C Significant differences in the cardinal growth temperatures and ammonium oxidation rates were observed using cells grown at 5 and 25 "C in chemostat culture. Cells grown at 5 "C had a n optimum growth temperature of 22 "C and a maximum of approximately 29 "C. Cells grown at 25C had an optimum growth temperature of 30 "C and a maximum of approximately 38 "C. Whole-cell kinetic studies failed to define the substrate of the enzyme system as either NH, or N f l , but did indicate that there are physiological differences between cells of this ammonium oxidizer when grown at 5 and 25OC. The data strongly suggest that temperatures close to the environmental temperatures should be used and that adaptation to higher temperatures should be avoided in autoecological studies.
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