EMBRYOGENESIS AND HAPLOID PLANT PRODUCTION FROM ANTHER CULTURE OF CABBAGE (<i>Brassica oleracea</i> var. <i>capitata</i> L.)

Abstract

Thirteen out of nineteen single plant cabbage (Brassica oleracea) selections from a clubroot-resistant breeding population with known variation in glucosinolate composition yielded embryos from cultured anthers. The highest embryo yield was obtained with a line that contained the lowest content of total glucosinolates and which was free of the glucosinolate hydrolysis product, goitrin. Of 191 regenerants examined from five selections, 23 haploids, 105 diploids, 162 tetraploids, and 1 aneuploid were identified.Key words: Tissue culture, glucosinolates, clubroot, cabbage, embryo, haploid