Immunocytolocalization of Plasma Membrane H⁺-ATPase

Abstract

The localization of plasma membrane H+-ATPase has been studied at the optical microscope level utilizing frozen and par- affin sections of Avena sativa and Pisum sativum, specific anti- ATPase polyclonal antibody, and second antibody coupled to alkaline phosphatase. In leaves and stems the ATPase is con- ' Present address: Instituto de Recursos Naturales y Agrobiologia, Avda. Reina Mercedes s/n,