Quantitative Determination of Sugars and Myo-Inositol in Citrus Fruits Grown in Japan Using High-Performance Anion-Exchange Chromatography

Abstract

Fifteen commercial samples of citrus fruits grown in Japan were analyzed for their sugar contents by high-performance anion-exchange chromatography (HPAEC) and electrochemical detection (ED) coupled with a stationary phase D10 column prepared using chloromethylated styrene-divinylbenzene copolymer and N,N,N',N'-tetramethyldiaminodecane. Myo-inositol, glucose, fructose, and sucrose in all of these various citrus fruits could be successfully separated within 25 min using 0.5 M NaOH eluent at a flow-rate of 0.4 mL/min. Myo-inositol, as a better nutritional source, was found in all the citrus fruits grown in Japan (0.7 +/- 0.04-2.1 +/- 0.2 g/L). The sugar contents of twelve citrus fruits grown in other countries were also determined.