TISSUE CULTURE AND REGENERATION OF DISTICHLIS SPICATA (GRAMINEAE)

Abstract

Distichlis spicata tissue cultures were initiated from mature seeds. Cultures displayed regenerable callus that was compact, white, and streaked with purple and green. Selection of compact areas at subculture established long‐term, regenerable cultures. Removal of auxin and inclusion of 1 mg 1 –1 BA in the maintenance media induced regeneration. Histological sections indicate that although cells were typical of embryogenic types, regeneration was by shoot organogenesis. Regenerated plants flowered and set seed under field conditions.