Chemoenzymatic Syntheses of Sialyloligosaccharides with Immobilized Sialidase

Abstract

The α‐glycosidic transfer of N ‐acetylneuraminic acid (Neu5Ac) to the terminally bound galactose of the acceptor molecule is achieved with immobilized sialidase obtained from Vibrio cholerae . This is the first application of this enzyme in the catalysis of transglycosylation, which produces (2–6)‐ and (2–3)‐linked sialyloligosaccharides. These molecules often cannot be prepared by feasible synthetic methods, yet their physiological importance in glycoproteins and ‐lipids continue to generate interest.