Science · 1994 · 834 citations · 41 references
Life SciencesImmunologyOncologyCancer ImmunologyImmunotherapyTumor ImmunologyAntigen RecognitionCancer ImmunotherapyHistocompatibility AntigensHuman Leukocyte Antigen SystemMajor Histocompatibility ComplexTumor AntigensAntigen Recognition By Cytotoxic T LymphocytesMhc-peptide RecognitionPeptide EpitopesT Cell Epitope RecognitionT-cell EpitopesTandem Mass SpectrometryTandem Mass Spectrometry AnalysisTumor-associated Antigen Recognition
Of several thousand peptides presented by the major histocompatibility molecule HLA-A2.1, at least nine are recognized by melanoma-specific cytotoxic T lymphocytes (CTLs). Tandem mass spectrometry was used to identify and to sequence one of these peptide epitopes. Melanoma-specific CTLs had an exceptionally high affinity for this nine-residue peptide, which reconstituted an epitope for CTL lines from each of five different melanoma patients tested. Recognition by multiple CTL lines suggests that this may be a promising candidate for use in peptide-based melanoma vaccines.
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Allele-specific motifs revealed by sequencing of self-peptides eluted from MHC molecules
Kirsten Falk, Olaf Rötzschke, Stefan Stevanovié et al. · Nature · 1991 · 2.4K citations
Prominent role of secondary anchor residues in peptide binding to HLA-A2.1 molecules
Jörg Ruppert, John Sidney, Esteban Celis et al. · Cell · 1993 · 632 citations