Proceedings of the National Academy of Sciences · 1971 · 203 citations · 11 references
Life SciencesInfectious DiseasesMolecular BiologyMolecular VirologyProtein TranslationVirologyViral InfectionsViral Rna BiologyViral TranscriptionVirus PurificationDouble-stranded Rna VirusesDouble-stranded Viral RnaViral Rna SynthesisViral Rna TranslationViral Translation Mechanisms
Infection of HeLa cells with poliovirus results in an inhibition of host-cell protein synthesis. Cytoplasmic extracts from infected cells contian an activity that inhibits initiation of protein synthesis in vitro by rabbit reticulocyte lysates. This inhibitory activity has been purified and characterized, and is shown to reside in poliovirus double-stranded RNA. The intact double-stranded molecule is not essential for inhibitory activity.
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