Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

F. William Studier, Barbara A. Moffatt · Journal of Molecular Biology · 1986 · 6.4K citations

Life Sciences, Microbiology, Molecular Biology +9

XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with beta-galactosidase selection.

William O. Bullock · Medical Entomology and Zoology · 1987 · 1.2K citations

Life Sciences, Biotechnology, Microbiology +13

The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels

K.F. Jensen · Journal of Bacteriology · 1993 · 514 citations

Life Sciences, Genetics, Microbiology +14

The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment

Thomas G.M. Schmidt, Arne Skerra · Protein Engineering Design and Selection · 1993 · 289 citations

Pharmacy, Biochemistry, Biotechnology +17