Rapid Communications in Mass Spectrometry · 2008 · 85 citations · 26 references
Natural SciencesMicrobiologyAnalytical ChemistryDiagnostic MicrobiologyMass Spectrometric AnalysisMass SpectrometryMass Spectrometry Data AnalysisPathogen DetectionAbundance PeaksMaldi-tof IdentificationMass Spectrometry IdentificationMass Spectrometry-based IdentificationMass Spectrometry-based Microbial IdentificationMatrix-assisted Laser Desorption Ionization Time-of-flight Mass SpectrometryMatrix-assisted Laser Desorption/ionizationMatrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral 'fingerprints' for twelve Penicillium species. Prior to MALDI-TOF MS analysis, eight replicate cultures of each Penicillium species were subjected to three one-minute bead-beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contained abundant peaks in the range of m/z 5000-20 000, and allowed unambiguous discrimination between species. In addition, a biomarker common to all Penicillium mass spectra was observed at m/z 13 900. Discriminant analysis using the MALDI-TOF MS data yielded classification error rates of 0% (i.e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance.
26
Monitoring the Growth of a Bacteria Culture by MALDI-MS of Whole Cells
Randy J. Arnold, Jonathan A. Karty, Andrew D. Ellington et al. · Analytical Chemistry · 1999 · 164 citations