International Journal of Systematic Bacteriology · 1997 · 82 citations · 14 references
Life SciencesMicrobiologyMolecular BiologyBacteriologyMicrobial EcologySoil Microbiology16S Ribosomal Ribonucleic Acid AnalysisBacterial SporesStrain Typing16S Ribosomal Ribonucleic Acid Gene Analysis16S Ribosomal Ribonucleic Acid Sequence Analysis16S Ribosomal Rna Gene AnalysisMolecular Identification Of StrainsReference StrainsSpore-forming Spoilage OrganismsThermophilic MicrobiologyThermophilic Microorganisms
The restriction enzyme profiles of 16S ribosomal DNAs (rDNAs) amplified by PCR from thermophilic heterotrophic bacterial strains isolated from composts were compared with those of reference strains. This allowed us to assign all but 1 of 16 strains to four different Bacillus species (namely, Bacillus stearothermophilus, Bacillus pallidus, Bacillus thermoglucosidasius, and "Bacillus thermodenitrificans"). This study showed that PCR restriction analysis of 16S rDNA contributes to rapid and reliable identification of newly isolated strains belonging to recognized species.
14
Identification of Bacillus Strains Using the API System
Niall A. Logan, R. C. W. Berkeley · Microbiology · 1984 · 446 citations