FEBS Letters · 1994 · 42 citations · 17 references
Life SciencesBiochemistryPlant BiologyEnzymologyPhotosynthesisPlant BiochemistryCatalytic MechanismsChloroplast BiochemistryEnzyme-substrate RecognitionPlant EnzymesRedox EnzymesThioredoxin SystemBinding Site TopographyChloroplast Thioredoxin SystemFerredoxin SystemsFerredoxin-dependent ReactionsFerredoxin-nadp+ Reductase InteractionRedox Regulation In PhotosynthesisThioredoxin Reductase SystemThioredoxin-dependent Enzymes
Differential chemical modification of acidic residues was used to map the binding site of plant ferredoxin (Fd) for the chloroplast enzyme ferredoxin:thioredoxin reductase (FTR). Binding of FTR to Fd inhibits chemical modification of Fd residues D34, D65, E92, E93, E94 and C-terminal A97. The binding site demarcated by these residues differs from that for ferredoxin:NADP+ reductase (FNR). The FTR site includes C-terminal residues but not helix 24-31, which is part of the FNR site. Both sites enclose the [2Fe-2S] cluster.
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