FEBS Letters · 1997 · 2.6K citations · 17 references
Life SciencesCell BiologyMolecular BiologyBiophotonicsGene ExpressionMolecular ImagingAnimal Cell CultureAnimal Cell Line DevelopmentBioluminescenceBioluminescence ImagingFluorescence MicroscopyFluorescence SpectroscopyAnimal BioluminescenceBioluminescent ReportersExcitationGenetically Encoded Fluorescent ReportersTransgenic Cell LinesTransgenic Mouse ModelsTransgenic Reporter LinesTransplantation
Green fluorescent protein (GFP) from jellyfish Aequorea victoria emits green light, and numerous mutants with altered spectra and higher extinction coefficients have been developed. We generated transgenic mice expressing an enhanced GFP (EGFP) driven by a chicken beta‑actin promoter and CMV enhancer. All tissues except erythrocytes and hair fluoresced green, making these cells useful for cell transplantation studies.
The green fluorescent protein (GFP) is responsible for the green bioluminescence of the jellyfish Aequorea victoria. Many classes of GFP mutants exist that display modified fluorescence spectra and an increased extinction coefficient. We produced transgenic mouse lines with an 'enhanced' GFP (EGFP) cDNA under the control of a chicken beta-actin promoter and cytomegalovirus enhancer. All of the tissues from these transgenic lines, with the exception of erythrocytes and hair, were green under excitation light. The fluorescent nature of the cells from these transgenic mouse lines would facilitate their use in many kinds of cell transplantation experiments.
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