Proceedings of the National Academy of Sciences · 2009 · 162 citations · 33 references
Life SciencesPlant BiologyPlant SciencesGene ExpressionPlant GeneticsPlant Molecular BiologyMicrorna BiogenesisMicrorna BiologyMicrorna-mediated RegulationPlant Gene ExpressionPlant Small Rna BiologyPlant Transcriptional RegulationArgonaute Protein FunctionCarpel DevelopmentDevelopmental Regulation By Small RnaHypomorphic AllelesHypomorphic Gene VariantsMicrorna In DevelopmentPhenotypic SimilarityVegetative Phase Change
Loss-of-function mutations of SQUINT (SQN)-which encodes the Arabidopsis orthologue of cyclophilin 40 (CyP40)-cause the precocious expression of adult vegetative traits, an increase in carpel number, and produce abnormal spacing of flowers in the inflorescence. Here we show that the vegetative phenotype of sqn is attributable to the elevated expression of miR156-regulated members of the SPL family of transcription factors and provide evidence that this defect is a consequence of a reduction in the activity of ARGONAUTE1 (AGO1). Support for this latter conclusion was provided by the phenotypic similarity between hypomorphic alleles of AGO1 and null alleles of SQN and by the genetic interaction between sqn and these alleles. Our results suggest that AGO1, or an AGO1-interacting protein, is a major client of CyP40 and that miR156 and its targets play a central role in the regulation of vegetative phase change in Arabidopsis.
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Specific Effects of MicroRNAs on the Plant Transcriptome
Rebecca Schwab, Javier F. Palatnik, Markus Riester et al. · Developmental Cell · 2005 · 1.5K citations · Full text
Widespread Translational Inhibition by Plant miRNAs and siRNAs
Peter Brodersen, Lali Sakvarelidze-Achard, M. Bruun-Rasmussen et al. · Science · 2008 · 1.5K citations