Changes in the actinomycetal communities during continuous thermophilic composting as revealed by denaturing gradient gel electrophoresis and quantitative PCR

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

Ligation-independent cloning of PCR products (LIC-PCR)

Charalampos Aslanidis, Pieter J. de Jong · Nucleic Acids Research · 1990 · 1.2K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +14

A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria

Soumitesh Chakravorty, Danica Helb, Michele Burday et al. · Journal of Microbiological Methods · 2007 · 1.2K citations · Full text

Life Sciences, Microbiology, Molecular Biology +16

Effect of genome size and rrn gene copy number on PCR amplification of 16S rRNA genes from a mixture of bacterial species

V. Farrelly, Fred A. Rainey, Erko Stackebrandt · Applied and Environmental Microbiology · 1995 · 723 citations

Life Sciences, Microbiology, Molecular Biology +15

Denaturing gradient gel electrophoresis (DGGE) in microbial ecology.

Gerard Muyzer, Thorsten Brinkhoff, Ulrich Nübel et al. · 2004 · 637 citations

Life Sciences, Microbiology, Molecular Biology +13