Marker-free plasmids for gene therapeutic applications—Lack of antibiotic resistance gene substantially improves the manufacturing process

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.2K citations

Life Sciences, Microbiology, Molecular Biology +11

A rapid alkaline extraction procedure for screening recombinant plasmid DNA

H.C. Birnboim, Janine Doly · Nucleic Acids Research · 1979 · 14.9K citations · Full text

Life Sciences, Genetics, Molecular Biology +11

Mass Spectrometric Sequencing of Proteins from Silver-Stained Polyacrylamide Gels

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Life Sciences, Biochemistry, Molecular Biology +17

Cyclic Adenosine Monophosphate in Bacteria

Ira Pastan, Robert L. Perlman · Science · 1970 · 505 citations

Life Sciences, Microbiology, Molecular Biology +15

Role of Inorganic Polyphosphate in Promoting Ribosomal Protein Degradation by the Lon Protease in <i>E. coli</i>

Akio Kuroda, Kazutaka Nomura, Ryo Ohtomo et al. · Science · 2001 · 372 citations

Life Sciences, Biochemistry, Microbiology +17