Somatostatin and Gastrin Release from Canine Stomach

Abstract

Somatostatin and gastrin release into the veins draining the stomach was studied in 27 anaesthetized dogs. Basal somatostatin-like immunoreactivity (SLI) in corpus veins (136 +/- 36 pg/ml) was significantly higher than in antrum veins (83 +/- 20 pg/ml; p less than 0.05) and the femoral artery (58 +/- 15 pg/ml; p less than 0.02). During peptone, pH 6.5, perfusion of the stomach, SLI concentration increased significantly in the corpus veins to approximately four times basal and in the antrum veins to three times basal, whereas SLI levels in the peripheral circulation remained constant. Peptone, pH 3.5, and sodium oleate did not stimulate gastric SLI. Gastric distension increased significantly SLI release from the corpus. In gel filtration studies 50%--70% of SLI from gastric vein plasma samples but greater than 90% from femoral artery samples eluted in the void volume of Sephadex G-25 columns. Gastrin secretion was stimulated significantly only by peptone, pH 6.5.