Determination of the complete genomic DNA sequence of <i>Thermoplasma volcanium</i> GSS1

Abstract

The complete genomic DNA sequence of the aero/anaero-facultative archaebacterium, Thermoplasma volcanium GSS1, has been determined. A number of DNA fragments were cloned by using the λ, cosmid, and BAC systems, and sequenced. The remaining 30 gaps were bridged by DNA fragments constructed using the polymerase chain reaction. The repetition in sequencing the same base positions was 13.1±7.5 fold. The alignment of the DNA fragments and the completeness of the genomic sequence were confirmed by the consistency of the genomic sequence with the lengths and partial sequences of a second set of DNA fragments that altogether covered 88% of the genome. The number of bases found in the genomic sequence is 1, 584, 799, with a G/C content of 39.9%. The combination of the four types of bases in the new genomic sequence is compared with those in known genomic sequences of similar sizes.