Publication | Closed Access
Genetically Encoded Cyclopropene Directs Rapid, Photoclick‐Chemistry‐Mediated Protein Labeling in Mammalian Cells
206
Citations
42
References
2012
Year
E. ColiMolecular BiologyClick ChemistryProtein SynthesisBiosynthesisProtein ExpressionCyclopropene Directs RapidPhotochemistryBiochemistryMechanistic PhotochemistryGenetic IncorporationPhotoclick ReactionPhotoclick‐chemistry‐mediated Protein LabelingMammalian CellsCell BiologyProtein BiosynthesisSignal TransductionNatural SciencesSynthetic BiologyMolecular SwitchPeptide SynthesisProtein EngineeringCellular BiochemistryMedicine
We just click: Genetic incorporation of a cyclopropene amino acid CpK (see scheme) site-specifically into proteins in E. coli and mammalian cells was achieved using an orthogonal aminoacyl-tRNA synthetase/tRNA(CUA) pair (CpKRS/MbtRNA(CUA)). Cyclopropene exhibited fast reaction kinetics in the photoclick reaction and allowed rapid (ca. 2 min) labeling of proteins.
| Year | Citations | |
|---|---|---|
Page 1
Page 1