Emerging infectious diseases · 2005 · 255 citations · 23 references
Life SciencesInfectious DiseasesMicrobiologyMedical MicrobiologyMolecular VirologyVirologyRespiratory InfectionsRespiratory Infectious DiseasesViral EpidemiologyViral GeneticsViral GenomicsViral InfectionsCoronavirus Respiratory InfectionPhylogenetic Character AnalysisPhylogenetic Diversity Analysis
The human coronavirus NL63 (HCoV-NL63) was first identified in The Netherlands, and its circulation in France has not been investigated. We studied HCoV-NL63 infection in hospitalized children diagnosed with respiratory tract infections. From November 2002 to April 2003, we evaluated 300 respiratory specimens for HCoV-NL63. Of the 300 samples, 28 (9.3%) were positive for HCoV-NL63. The highest prevalence was found in February (18%). The main symptoms were fever (61%), rhinitis (39%), bronchiolitis (39%), digestive problems (33%), otitis (28%), pharyngitis (22%), and conjunctivitis (17%). A fragment of the spike protein gene was sequenced to determine the variety of circulating HCoV-NL63. Phylogenetic analysis indicated that strains with different genetic markers cocirculate in France.
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A Novel Coronavirus Associated with Severe Acute Respiratory Syndrome
Dean D. Erdman, Goldsmith Cs, Zaki et al. · New England Journal of Medicine · 2003 · 4.5K citations
R. P. Singh, Jolanta Kurz, Gilles Boiteau · Journal of Virological Methods · 1996 · 2.6K citations