Polynucleotide-Protein Interactions in the Translation System. Detection of Contacts between Some Ribosomal Split Proteins and 16-S RNA in 30-S Subunits of Escherichia coli Ribosomes

Abstract

Direct contacts between 16-S RNA and split proteins S2, S3, S5, S14 and S21 inside the 30-S subunit of Escherichia coli ribosomes were evidenced by the formation of ultraviolet-induced (lambda = 254 nm) RNA-protein cross-links. 30-S subunits were reassembled from core particles and a mixture of split proteins containing in each case a single 125I-labelled protein. All the proteins tested are cross-linked as a result of a single-hit process; proteins S3 and S21 were cross-linked at the highest rate.