Nanotechnology · 2010 · 42 citations · 50 references
PharmacyBiochemistryCancer BiologyImmunotechnologyMolecular ImagingTheranosticsAntibody ConjugatesAntibody ConjugationCancer NanomedicineNanomedicineQuantum Dots In BiologyTheranostic NanoparticlesBiomolecular DetectionCore-shell Quantum DotsFluorescence Polarization ImagingFluorescence Polarization MicroscopyHepatocellular Carcinoma ImagingNanoparticle ConjugationNanoparticle Imaging AgentsQuantum Dot Imaging
We report the imaging of hepatocellular carcinoma cells and the immunoassay for alpha fetoprotein (AFP) using CdTe/CdS/ZnS core-shell-shell QDs. Stable and high PLQY (20%-48%) CdTe/CdS/ZnS core-shell-shell QDs were synthesized by a stepwise process. Bioconjugation of the core-shell-shell QDs with streptavidin (SA) was successfully applied in immunofluorescent imaging of the human hepatocellular carcinoma (HCC) cell line HepG2.2.15. Furthermore, the thioglycolic acid (TGA)-capped CdTe/CdS/ZnS core-shell-shell QDs fluorescence lifetime is longer than fluorescein, so it was first engaged to conjugate with antigen for the determination of protein (AFP) by fluorescence polarization immunoassay.
50
Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
Warren C. W. Chan, Shuming Nie · Science · 1998 · 7.2K citations
In vivo cancer targeting and imaging with semiconductor quantum dots
Xiaohu Gao, Yuanyuan Cui, Richard M. Levenson et al. · Nature Biotechnology · 2004 · 4.7K citations
Xiaogang Peng, M. C. Schlamp, A. V. Kadavanich et al. · Journal of the American Chemical Society · 1997 · 2.4K citations
Natural Sciences, Materials Engineering, Materials Science +17