Carbon and Nitrogen Analysis of Soil Fractions Using Near‐Infrared Reflectance Spectroscopy

Abstract

Abstract A nondestructive method to determine total C and N concentrations in soil size fractions is desirable when a limited sample is available. Near‐infrared reflectance spectroscopy (NIRS) was used to determine the total C and N concentrations in silt (50.0–2.0 µm) and coarse clay (2.0–0.2 µm), separated from 12 surface soils, by regressing the diffuse reflectance of near‐infrared radiation with constituent concentrations determined using combustion techniques. The correlation coefficients ( R 2 ) of the calibration equations were 0.93 for C and 0.89 for N, and the standard errors associated with NIRS predictions were 6.2 g kg −1 soil for total C and 0.6 g kg −1 for total N. Equation development with only silt samples improved the accuracy of NIRS calibration equations. Coefficients of variation [CV = (standard error of performance ÷ mean of the combustion procedure) × 100] for validation sample sets ranged from 14 to 19%, which is within the acceptable range for determining inorganic elements in plant tissues. We conclude that NIRS can be used to predict C and N concentrations in soil size fractions.