Science · 1963 · 141 citations · 4 references
Natural SciencesBiochemistryChemistryAnalytical ChemistryBiosensorsSpectroscopyBioluminescenceCalcium-binding ProteinsFluorescence SpectroscopyOptical BiosensingOptical BiosensorsBioluminescence QuantificationBioluminescent ProbesCalcium BiochemistryFluorescent Calcium IndicatorsJellyfish BiologyLuminescence-based AssaysLuminescent Sensors
A bioluminescent protein, aequorin, isolated from the jellyfish Aequorea in dilute disodium ethylenediaminetetraacetate solution, emits light on addition specifically of Ca(++) or Sr(++), thus providing the basis for a simple, quantitative micromethod for the determination of these cations, especially in biological fluids.
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