Concepedia

Spontaneous α-N-6-Phosphogluconoylation of a “His Tag” inEscherichia coli:The Cause of Extra Mass of 258 or 178 Da in Fusion Proteins

Kieran F. Geoghegan, H. B. F. Dixon, Philip J. Rosner, Lise R. Hoth, Anthony J. Lanzetti, Kris A. Borzilleri, Eric S. Marr, Linda H. Pezzullo, LeRoy Martin, Peter K. LeMotte,

Analytical Biochemistry · 1999 · 219 citations · 16 references

DOI

Concepts

References

16

New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

Erich Hochuli, Heinz Döbeli, Alfred Schacher · Journal of Chromatography A · 1987 · 1.1K citations

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Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

Erich Hochuli, Willi Bannwarth, Heinz Döbeli et al. · Nature Biotechnology · 1988 · 1.1K citations

, , +17

Current protocols in protein science

John L. Howland · Biochemical Education · 1996 · 468 citations

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Detailed peptide characterization using PEPTIDEMASS – a World‐Wide‐Web‐accessible tool

Marc R. Wilkins, Ingrid Lindskog, Elisabeth Gasteiger et al. · Electrophoresis · 1997 · 367 citations

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Purification of his-tagged proteins in non-denaturing conditions suggests a convenient method for protein interaction studies

Alexander Hoffmann, Robert G. Roeder · Nucleic Acids Research · 1991 · 290 citations · Full text

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