Influence de la teneur en gaz carbonique sur la morphogenèse de la vigne en culture <i>in vitro</i>

Abstract

Two different types of morphogenesis are induced in vitro in the grapevine by changing the system of sealing the culture tubes (with or without Parafilm). The differences appear mainly in the degree of miniaturiation: the plants the most miniaturized, those with Parafilm, have all the characteristics of an immature juvenile state. The others, without Parafilm, show an intermediate morphology between the adult and the juvenile forms. It follows then that the importance of juvenile characters is linked to the degree of miniaturization. One of the effects of Parafilm is in the modification of the amount of CO 2 in the atmosphere of the tubes: in excess during the dark phase and in severe shortage during the light phase. We have found then, that among all the environmental factors which could influence the morphogenesis of the grapevine in vitro, CO 2 appears to play a relatively important role.