70 S Ribosomes Bind to Shine–Dalgarno Sequences without Required Dissociations

Abstract

To dissociate or not to dissociate. Although it is thought that the intact 70S ribosome must dissociate into 30S and 50S subunit and the dissociated 30S subunit binds to a Shine–Dalgarno (SD) sequence of mRNA in the first step of the initiation translation, we found an additional pathway that the intact 70S ribosome can bind directly to mRNA SD sequences without a required dissociation.