Reproduction · 1994 · 21 citations · 13 references
Red deer hinds (n = 38) were treated in the breeding season with five different gonadotrophin regimens to investigate the temporal relationship between oestrus, ovulation and the LH surge. All hinds were treated with progesterone-impregnated controlled internal drug release (CIDR) devices to synchronize oestrus. The five treatments were as follows: treatment 1, controls; treatments 2, 3 and 4, 1200 iu pregnant mares' serum gonadotrophin (PMSG) was administered i.m. 72 h before CIDR device withdrawal (treatments 3 an 4 were also injected i.v. with 0.4 mg synthetic GnRH 12 or 18 h after CIDR device withdrawal, respectively); treatment 5, 200 iu PMSG was administered i.m. 72 h before CIDR device withdrawal and 0.5 iu FSH was administered in eight equal doses at intervals of 12 h starting from the time of PMSG injection. The hinds were run with crayon-harnessed stages to determine the time of oestrus onset. Blood samples were collected every 2 days for 26 days after CIDR device removal to determine concentrations of plasma progesterone and every 2 h for 72 h after CIDR device removal to determine plasma LH profiles. Laparoscopy for ovary examination was performed 6 or 12 h after oestrus onset and was repeated twice at intervals of 12 h. Final ovulation rate was determined on day 7 after CIDR device removal. All hinds received 500 micrograms cloprostenol i.m. on day 13. A total of 30 and 34 hinds exhibited oestrus and ovulation, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)
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