Simultaneous Determination of Fusidic Acid, m‐ and p‐Hydroxybenzoates and Butylhydroxyanisole by TLC with Densitometric Detection in UV

Abstract

Abstract A thin‐layer chromatography (TLC)‐densitometric method has been developed for the identification and quantification of fusidic acid (FA) (RF=0.53), methyl hydroxybenzoate (MHB) (RF=0.64), propyl hydroxybenzoate (PHB) (RF=0.72), and butylated hydroxyanisol (BHA) (RF=0.77), which are the components of a dermatological ointment. To separate these constituents, n‐hexane‐ethyl acetate‐glacial acetic acid (6∶3∶1, v/v/v) has been used as the mobile phase and silica gel 60 F254 TLC plates as the stationary phase. To detect the spots on chromatograms, densitometric measurements at three different wavelengths were carried out, i.e., 240 nm (FA), 260 nm (MHB, PHB), and 290 nm (BHA), leading to increased selectivity and decreased interferences of the peaks. The results of the model drug examination were characterized by good precision, accuracy, and high sensitivity.