Acta Crystallographica Section D Biological Crystallography · 2004 · 10 citations · 12 references
Life SciencesBiochemistryChemistryAnalytical ChemistryEnzymologyPlant Molecular BiologyCrystallization AnalysisCrystallization ProcessesCrystallographyEnzyme CharacterizationStructural EnzymologyX-ray CrystallographyCarbohydrate CrystallographySynchrotron DiffractionSynchrotron X-ray DiffractionUnit Cell ParametersX-ray Diffraction Analysis
Fructan 1-exohydrolase IIa (1-FEH IIa), a plant enzyme involved in fructan breakdown, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals are tetragonal, belonging to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = 139.83, b = 139.83, c = 181.94 A. Calculation of the Matthews coefficient indicates there to be two or three molecules in the asymmetric unit. Synchrotron radiation was used to collect a complete native data set to a resolution of 2.35 A.
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