Dimethyl Sulfoxide Has an Impact on Epigenetic Profile in Mouse Embryoid Body

TLDR

DMSO is a widely used solvent and cryopreservant that can alter cell fate, likely by perturbing epigenetic regulation of development. The study employed RT‑PCR to examine DMSO’s effects on DNA methyltransferases and histone‑modifying enzymes in mouse ESCs and embryoid bodies, and used restriction landmark genomic scanning to map locus‑specific DNA methylation changes. DMSO (0.02–1%) upregulated Dnmt3a expression and protein levels, induced hypermethylation of repetitive elements, and produced genome‑wide DNA‑methylation alterations associated with phenotypic changes in embryoid bodies.

Abstract

Dimethyl sulfoxide (DMSO), an amphipathic molecule, is widely used not only as a solvent for water‐insoluble substances but also as a cryopreservant for various types of cells. Exposure to DMSO sometimes causes unexpected changes in cell fates. Because mammalian development and cellular differentiation are controlled epigenetically by DNA methylation and histone modifications, DMSO likely affects the epigenetic system. The effects of DMSO on transcription of three major DNA methyltransferases (Dnmts) and five well‐studied histone modification enzymes were examined in mouse embryonic stem cells and embryoid bodies (EBs) by reverse transcription‐polymerase chain reaction. Addition of DMSO (0.02%–1.0%) to EBs in culture induced an increase in Dnmt3a mRNA levels with increasing dosage. Increased expression of two subtypes of Dnmt3a in protein levels was confirmed by Western blotting. Southern blot analysis revealed that DMSO caused hypermethylation of two kinds of repetitive sequences in EBs. Furthermore, restriction landmark genomic scanning, by which DNA methylation status can be analyzed on thousands of loci in genic regions, revealed that DMSO affected DNA methylation status at multiple loci, inducing hypomethylation as well as hypermethylation depending on the genomic loci. In conclusion, DMSO has an impact on the epigenetic profile: upregulation of Dnmt3a expression and alteration of genome‐wide DNA methylation profiles with phenotypic changes in EBs.