Proceedings of the National Academy of Sciences · 1984 · 138 citations · 38 references
Life SciencesMolecular BiologyMolecular GeneticsGene ExpressionTranscriptionTranscriptional RegulationPromoter RegulationRna TranscriptionTranscription InitiationViral Gene ExpressionViral Rna BiologyViral TranscriptionAdenovirus BiologyHerpesvirus TranscriptionPromotersTranscriptional Regulation Of Viral GenesViral Gene Expression RegulationViral PromotersViral Rna TranscriptionViral Transcriptional Regulation
Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids containing the viral EIIa or EIII transcription units. The amount of RNA produced from the EIIa and EIII promoters was increased by the products of both the 13S and the 12S RNAs. By measuring the level of specific transcription in nuclei isolated from transfected cells we directly demonstrate that the increased amount of EIIa RNA is due to stimulation of the rate of transcription.
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