The Journal of Experimental Medicine · 1987 · 33 citations · 12 references
Life SciencesCell BiologyImmunologyAutoimmunityMolecular ImmunologyNeuroimmunologyCentral Nervous System ImmunityCerebrospinal Fluid ImmunologyImmune SignalingNatural Killer Cell BiologyT Cell BiologyT Cell Receptor SignalingCerebrospinal Fluid Immune Cell PhenotypingCerebrospinal Fluid ImmunophenotypingCerebrospinal Fluid SystemIntracellular Calcium DynamicsT Cell Receptor StructureT-cell Receptor Clonality
We have derived 33 independent T cell clones from the cerebrospinal fluid (CSF) of a patient with subacute sclerosing panencephalitis using a single T cell cloning method. 6% (2 of 33) of these clones express the T cell receptor gamma (TCR-gamma) protein and are called CSF TCR-gamma clones. Phenotypic analyses of the CSF TCR-gamma clones indicate that they are WT-31-, CD3+, CD4-, and CD8-. The TCR-gamma protein exists on the cell surface as part of an 85-kD disulphide-linked dimer noncovalently associated with the CD3 polypeptides. The CSF TCR-gamma clones have NK-like activity that can be inhibited by anti-CD3 mAbs. Both CSF TCR-gamma clones proliferated in response to anti-CD3 mAbs coupled to Sepharose beads and/or IL-2. Furthermore, stimulation of one of these clones with anti-CD3 mAbs results in a rapid rise in intracellular calcium. These data suggest that T cells bearing the CD3-TCR-gamma protein complex are functional and play a role in the human immune response.
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Identification of a putative second T-cell receptor
Michael B. Brenner, Joanne McLean, Deno P. Dialynas et al. · Nature · 1986 · 990 citations
A T-cell receptor γ/CD3 complex found on cloned functional lymphocytes
Jannie Borst, R J van de Griend, J W van Oostveen et al. · Nature · 1987 · 443 citations