High Contrast in Vitro and in Vivo Photoluminescence Bioimaging Using Near Infrared to Near Infrared Up-Conversion in Tm³⁺ and Yb³⁺ Doped Fluoride Nanophosphors

TLDR

The authors synthesized 20–30 nm NaYF₄ nanocrystals co‑doped with Tm³⁺ and Yb³⁺ that exhibit NIR‑to‑NIR up‑conversion, enabling deep tissue penetration and high‑contrast imaging without autofluorescence or significant scattering. In vitro, the nanophosphors were taken up by cells and emitted at ~800 nm under 975 nm excitation with no cytotoxicity, and in vivo Balb‑c mice injected intravenously displayed high‑contrast photoluminescence imaging.

Abstract

A new approach for photoluminescence imaging in vitro and in vivo has been shown utilizing near infrared to near infrared (NIR-to-NIR) up-conversion in nanophosphors. This NIR-to-NIR up-conversion process provides deeper light penetration into biological specimen and results in high contrast optical imaging due to absence of an autofluorescence background and decreased light scattering. Aqueous dispersible fluoride (NaYF4) nanocrystals (20-30 nm size) co-doped with the rare earth ions, Tm(3+) and Yb(3+), were synthesized and characterized by TEM, XRD, and photoluminescence (PL) spectroscopy. In vitro cellular uptake was shown by the PL microscopy visualizing the characteristic emission of Tm(3+) at approximately 800 nm excited with 975 nm. No apparent cytotoxicity was observed. Subsequent animal imaging studies were performed using Balb-c mice injected intravenously with up-converting nanophosphors, demonstrating the high contrast PL imaging in vivo.