Comparison of the TLDA with the Nanodrop and the reference Qubit system

Abstract

The TLDA (transmitted light drop analyser) is a new microvolume UV-visible drop spectrophotometer new to the market. Assays were compared between a TLDA, NanoDrop and the Qubit system which is based on the gold standard for DNA assay, PicoGreen. The evaluation was carried out by the Trinity Biobank in St. James Hospital, Dublin on Buccal swabs and Blood samples. The data is discussed in this paper. The Qubit system is seen as the reference method in most studies as this is believed to provide more accurate results than UV absorbance methods because it distinguishes between DNA, RNA, free nucleotides, and other contaminants. The Qubit system uses fluorescent dyes to measure the concentration of the molecule of interest. The results compare both the spectrophotometric methods against the Qubit fluorescence technique.