Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE · 2006 · 13 citations · 0 references
EngineeringMolecular Recognition ElementsPathogen DetectionMicrobial PathogensBiomolecular ToolMolecular BiologyNucleic Acid Amplification TestBacterial PathogensCapillary Electrophoretic AnalysisBioanalysisFood MicrobiologyAnalytical ChemistryMolecular DiagnosticsOther Food PathogensHost-pathogen InteractionsChromatographyCapillary ElectrophoresisVirulence FactorFoodborne PathogensBiomedical AnalysisPathogen CharacterizationMolecular MicrobiologyClinical MicrobiologyBiomolecular EngineeringRelative Binding AffinityNucleic Acid AmplificationMicrobiologyMedicineDna Aptamer
The biomolecular interactions between a fluorescently labeled aptamer and whole cell<i> Campylobacter jejuni</i>(<i>C. jejuni</i>) have been characterized using capillary electrophoresis with laser-induced fluorescence detection. From electrophoretic analysis, the bound complex forms, unbound aptamer, and cells were visualized. The relative binding affinity of the DNA aptamer with <i>C. jejuni </i>was compared with other food-borne pathogens including <i>Escherichia coli O157:H7 </i>and <i>Salmonella typhimurium</i>. Preliminary data suggests that this aptamer exhibits strong binding affinity towards <i>C. jejuni </i>with minimal cross reactivity over other food pathogens when equivalent cell concentrations were used.