Canadian Journal of Biochemistry · 1964 · 41 citations · 8 references
PharmacyBiochemistryMolecular BiologyBiophysical ChemistryNucleic Acid BiochemistryNucleic Acid ChemistryNucleic Acid ModificationNucleic Acid StructureOligonucleotide ChemistryRibonucleic Acid (Rna) ChemistryRna ChemistryRna Structure2'-O-methoxyethyl Oligonucleotides2'-O-methyl Modification2'-O-methyl Oligonucleotides2'-O-methyl RnaAlkali TreatmentOligonucleotide StabilityReactivityRibonucleotide Chemistry
Methods are described which are suitable for the quantitative analysis of the alkali-stable oligonucleotides which result from the hydrolysis of ribonucleates in molar alkali at room temperature for 90 hours. The alkali-stability of the oligonucleotides is presumed to result from 2′-O-methyl substitution of ribose residues and this assumption is supported by the relatively slight reactivity of the purine 2′-O-methyl ribosides in the orcinol color reaction for pentoses. The existence of all of the 16 possible dinucleotides as part of ribonucleate chains has been established. The quantity of alkali-stable trinucleotides is much greater than would be expected for a random spatial distribution of 2′-O-methyl ribosides in ribonucleate chains.
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Methods for Studying Nucleotide Metabolism in Synchronized Cultures of Protozoa.
Paul Plesner, Carl‐Henric de Verdier, John Glomset et al. · Acta chemica Scandinavica/Acta chemica Scandinavica. B, Organic chemistry and biochemistry/Acta chemica Scandinavica. A, Physical and inorganic chemistry/Acta chemica Scandinavica. Series B. Organic chemistry and biochemistry/Acta chemica Scandinavica. Series A, Physical and inorganic chemistry · 1955 · 71 citations · Full text
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