Three-dimensional location of fluorescent inhomogeneities in turbid media by scanning heterodyne holography

Abstract

We describe and illustrate experimentally a method aimed at the three-dimensional (3-D) imaging of fluorescent inhomogeneities embedded in a turbid medium. The method utilizes incoherent scanning holography to capture 3-D information in a single two-dimensional scan and phase-sensitive heterodyne detection to reject multiply scattered light and to produce a single-sideband holographic record. The 3-D imaging capability of the method is illustrated by an example.