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CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice

Julie Thompson, Desmond G. Higgins, Toby J. Gibson · Nucleic Acids Research · 1994 · 64.9K citations · Full text

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One-step inactivation of chromosomal genes in <i>Escherichia coli</i> K-12 using PCR products

Kirill A. Datsenko, Barry L. Wanner · Proceedings of the National Academy of Sciences · 2000 · 15.2K citations · Full text

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Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

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ESPript: analysis of multiple sequence alignments in PostScript.

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Vienna RNA secondary structure server

Ivo L. Hofacker · Nucleic Acids Research · 2003 · 2.4K citations · Full text

Life Sciences, Bioinformatics, Molecular Biology +15