Proceedings of the National Academy of Sciences · 2001 · 458 citations · 21 references
AgriculturePlant BiologyPlant SciencesHerbicide ResistancePlant BiochemistryPlant GeneticsCellulose Biosynthesis InhibitionHerbicide Resistance MechanismsHerbicide ToleranceMolecular Basis Of ResistancePlant EnzymesResistance Gene FunctionCellulose Biosynthesis Herbicide ResistanceCellulose Synthase Gene FamiliesCellulose Synthase InhibitionCellulose Synthesis InhibitionCloningHerbicide Resistance GenesMutation-driven Herbicide ResistancePoint Mutations
In many higher plants, cellulose synthesis is inhibited by isoxaben and thiazolidinone herbicides such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl) phenyl-4-thiazolidinone. Semidominant mutations at the IXR1 and IXR2 loci of Arabidopsis confer isoxaben and thiazolidinone resistance. Isolation of the IXR1 gene by map-based cloning revealed that it encodes the AtCESA3 isoform of cellulose synthase. The two known mutant alleles contain point mutations that replace glycine 998 with aspartic acid, and threonine 942 with isoleucine, respectively. The mutations occur in a highly conserved region of the enzyme near the carboxyl terminus that is well separated from the proposed active site. Although the IXR1 gene is expressed in the same cells as the structurally related RSW1 (AtCESA1) cellulose synthase gene, these two CESA genes are not functionally redundant.
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Molecular Analysis of Cellulose Biosynthesis in <i>Arabidopsis</i>
Tony Arioli, Liangcai Peng, Andreas S. Betzner et al. · Science · 1998 · 818 citations