Endocrinology · 1992 · 10 citations · 8 references
MedicineEndocrinologyMolecular BiologyGene ExpressionMolecular OncologySignal TransductionNuclear Receptor SignalingNuclear ReceptorsSteroid Hormone SignalingThyroid CancerThyroid DisordersThyroid EndocrinologyAdenylate Cyclase RegulationAdenylate Cyclase SignalingNeoplastic DiseaseNuclear Hormone ReceptorsNuclear Thyroid Hormone SignalingThyroid AdenomasThyroid Cancer PathologyThyroid Hormone Receptors
The expression of beta 2-adrenergic receptor (AR) mRNA was investigated in normal and neoplastic human thyroid tissues. A combination of techniques for reverse transcribing mRNA into cDNA and the incorporation of 32P-gamma ATP into the polymerase chain reaction (PCR)-generated fragments allowed us to detect beta 2-AR mRNA in surgically excised thyroid specimens. The levels of beta 2-AR cDNA generated by PCR in thyroid adenomas and cancers were 3.3 and 6.9 times, respectively, as high as that of normal thyroid tissues. These findings suggest that the level of beta 2-AR mRNA is correlated with the extent of differentiation in neoplastic tissues. The present study provides new insights into the relationships between the AR-adenylate cyclase system and the regulation of the growth and differentiation in neoplastic human thyroid tissues.
8
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.2K citations
Two G Protein Oncogenes in Human Endocrine Tumors
John F. Lyons, Claudia A. Landis, Griffith Harsh et al. · Science · 1990 · 1K citations