Heterologous transplantation of human hematopoietic cell lines

Abstract

Heterologous transplantation of 15 human lymphocytoid cell lines into sublethally irradiated mice was studied. All of the 15 lines were used for subcutaneous transplantation, but only 10 were used for intraperitoneal transplantation. When 107 viable cells were inoculated subcutaneously, 11 of the 15 lines produced subcutaneous tumors in irradiated mice. When 107 cells were inoculated intraperitoneally, 2 lines derived from patients with myelogenous leukemia produced either solid tumors or ascites; 3 other cell lines produced only a solid tumor at the site of injection. Cells recultured from the transplants were essentially identical to the original inoculated cells in chromosome constitution, cloning efficiency, and production of immunoglobulins. There was some correlation of heterotransplantability with abnormal chromosome constitution and higher cloning efficiencies.