Bioscience Biotechnology and Biochemistry · 2006 · 15 citations · 20 references
Life SciencesBiochemistryMolecular BiologyCellular MetabolismGene ExpressionMetabolismAmino Acid MetabolismProtein SynthesisYeast BiologyYeast GeneticsAmino AcidsType Strain CharacterizationYeast Gene Expression RegulationYeast Gene FunctionYeast Gene RegulationYeast MutagenesisYeast Strain Engineering
We characterized a trifluoroleucine-resistant mutant of Saccharomyces cerevisiae, TFL20, that has a mutation in the LEU4 gene. We monitored the concentration of extracellular i-AmOH and intracellular amino acids, and compared the ratios of gene expression in TFL20 with the wild-type strain, K30. We found that the LEU1, LEU2, and BAT1 genes were up-regulated in TFL20 for metabolism, and that TFL20 simultaneously produced as much i-AmOH and leucine as K30 does.
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Elizabeth I. Boyle, Shuai Weng, Jeremy Gollub et al. · Bioinformatics · 2004 · 2.2K citations · Full text