Conjugated Linoleic Acids Attenuate <scp>LPS</scp>‐Induced Pro‐Inflammatory Gene Expression by Inhibiting the <scp>NF</scp>‐κB Translocation Through <scp>PPAR</scp>γ in Buffalo Granulosa Cells

Abstract

Problem In granulosa cells, TLR 4‐mediated LPS ‐induced immune response interferes with ovarian granulosa cell function. Method of study LPS ‐induced pro‐inflammatory gene expression was monitored by real‐time PCR and NF ‐κB was determined by the immuno‐blotting and immuno‐staining in granulosa cells in vitro . Results The LPS (1 μg/mL) increased pro‐inflammatory gene expression which was reverted back by treatment with TLR 4 signaling inhibitor. Cotreatment of CLA (10 μ m ) with LPS did not show any effect on LPS ‐induced pro‐inflammatory gene expression but granulosa cells pre‐treated with CLA for 24 hr, attenuated LPS ‐induced pro‐inflammatory gene expression and nuclear NF ‐κB. GW 9662, a PPAR γ‐inhibitor, further increased the expression of pro‐inflammatory genes. Conclusion The present findings reiterated that pre‐treatment with CLA can prevent LPS ‐induced granulosa cells dysfunction.